2026-09-24T20:39:07Z https://epublications.vu.lt/oai

oai:vu.lt:elaba:76894409 2026-07-09T00:43:17Z openaireNDLTDDRIVERDART-EUROPE

Su onkologinėmis ligomis siejamas pre-iRNR splaisingas: splaisingo veiksnių ir hipoksinės mikroaplinkos tyrimas /Pre-mRNA splicing associated with oncological diseases: a study of splicing factors and hypoxic microenvironment.Pečiulienė, Inga,info:eu-repo/semantics/openAccessinfo:eu-repo/semantics/altIdentifier/doi/10.15388/vu.thesis.104dissertation ; hypoxia ; cancer ; pre-mRNA ; splicingOncological diseases remain one of the unsolved problems of the entire society as the oncology-related morbidity and mortality has been systematically increasing. As pre-mRNA splicing is a highly important process in regulating gene expression, it is not surprising that changes in splicing have been observed in many different diseases. Unfortunately, the reasons for the expression of disease-associated mRNA isoforms and their regulatory factors have not been completely understood yet. This study describes the research of splicing factors and the expression of tumor-associated mRNA isoforms in healthy and tumor gastrointestinal organ tissues and tumors cell lines, and examines whether/how hypoxic microenvironment in tumor cell lines affects tumor-associated pre-mRNA splicing changes. Studies in this work have shown differences in the expression of SRSF1, SRSF2, U2AF65, U2AF35 and KHSRP splicing factors and tumor-associated Fas and Rac1 mRNA isoforms in tumor tissues and commonly used tumor cell lines. Our results indicate that reduced oxygen content in the cell environment (hypoxia) affects Fas pre-mRNA alternative splicing. The results obtained in this work provide valuable information about the processes initiating diseases in various systems.Institutional Repository of Vilnius Universityinfo:eu-repo/semantics/doctoralThesislit2020application/pdfhttps://epublications.vu.lt/object/elaba:76894409/76894409.pdfhttps://repository.vu.lt/VU:ELABAETD76894409&prefLang=en_US