| Abstract [eng] |
S. cerevisiae and other yeast species of the Saccharomyces genus possess endogenous ssRNA and dsRNA viruses. The best-known are killer phenotype-encoding LA and M dsRNA viruses of the Totiviridae family. In nature, M viruses are detected altogether with their specific helper virus LA. The interplay between LA viruses and M viruses and the specificity determinants are not fully understood. The research of the mentioned aspects is essential for understanding of the biocidic phenotype of yeasts and revealing the ontogenesis mechanism of these viruses. This study evaluated the impact of recombinant LA viral protein expression on all known S. cerevisiae LA and M viral systems and related, belonging to the S. paradoxus viral system. It was found that the expression of truncated LA capsid protein Gag causes the elimination of native yeast viruses despite their type. For the first time, the interactions of LA-1, LA-lus, and LA-28 viral proteins with native viral systems were studied systematically. The results indicate that the specificity of interactions between recombinant LA proteins and endogenous yeast viral systems could depend on the specific type of M virus. The role of variable sequences of LA viral proteins in establishing interactions between recombinant proteins and endogenous LA and M viruses was assessed. |
