| Abstract [eng] |
Cancer remains one of the world’s greatest health problems, causing millions of deaths every year, and requiring new treatment strategies. The protein carbonic anhydrase IX (CA IX) is an attractive target for the development of new anticancer drugs because it is found in various types of solid tumors. In addition to its primary function, of catalyzing the reversible carbon dioxide hydration reaction, CA IX is also involved in cancer cell metastasis. Therefore, the effect of the inhibitor VD11-4-2, which has a high affinity for CA IX, on breast cancer cell migration was investigated in this dissertation. The studies were performed by tracking the movement of individual cells, assessing their speed and movement direction. This study model was used to investigate the anti-migratory effects of CA IX inhibitors for the first time. The application of inhibitor VD11-4-2 as a CA IX targeting moiety in a nanosystem was also investigated in this dissertation. CA IX is an attractive destination for targeted drug delivery systems because it is a transmembrane protein with an active center located on the outer side of the cell. VD11-4-2 was attached to the surface of porous silica and zinc-copper oxide-based nanoparticles and evaluated for the binding to CA IX as well as for their anticancer effect towards breast cancer cells. VD11-4-2 shows fluorescence properties, which enabled monitoring cancer cell uptake and payload release of drug-loaded porous silicon nanoparticle-based nanosystem. Overall, the work described in the dissertation highlights the advantages of CA IX as an anticancer drug target and expands the possible applications of the inhibitor VD11-4-2. |
