| Title |
Selective immunocapture and light-controlled traceless release of transiently caged proteins / |
| Authors |
Rakauskaitė, Rasa ; Urbanavičiūtė, Giedrė ; Simanavičius, Martynas ; Žvirblienė, Aurelija ; Klimašauskas, Saulius |
| DOI |
10.1016/j.xpro.2021.100455 |
| Full Text |
|
| Is Part of |
STAR protocols.. Cambridge, MA : Cell Press. 2021, vol. 2, iss. 2, art. no. 100455, p. [1-12].. eISSN 2666-1667 |
| Keywords [eng] |
Antibody ; Molecular/Chemical Probes ; Protein Biochemistry |
| Abstract [eng] |
The 4,5-dimethoxy-2-nitrobenzyl (DMNB) photocaging group introduced into small biomolecules, peptides, oligonucleotides, and proteins is commonly used for spatiotemporal control of chemical and biological processes. Here, we describe the use of a DMNB-selective monoclonal antibody for non-covalent capture of chemically or biosynthetically produced proteins containing surface-exposed DMNB caging groups followed by light-controlled traceless decaging and release of the bound proteins into solution for a variety of downstream applications. For complete details on the use and execution of this protocol, please refer to Rakauskaitė et al. (2020). |
| Published |
Cambridge, MA : Cell Press |
| Type |
Journal article |
| Language |
English |
| Publication date |
2021 |
| CC license |
|
