Title |
Human polymerase δ-Interacting protein 2 (PolDIP2) inhibits the formation of human Tau oligomers and fibrils / |
Authors |
Kasho, Kazutoshi ; Krasauskas, Lukas ; Smirnovas, Vytautas ; Stojkovič, Gorazd ; Morozova-Roche, Ludmilla A ; Wanrooij, Sjoerd |
DOI |
10.3390/ijms22115768 |
Full Text |
|
Is Part of |
International journal of molecular sciences.. Basel : MDPI. 2021, vol. 22, no. 11, art. no. 5768, p. [1-14].. eISSN 1422-0067 |
Keywords [eng] |
PolDIP2 ; Tau ; amyloid |
Abstract [eng] |
A central characteristic of Alzheimer's disease (AD) and other tauopathies is the accumulation of aggregated and misfolded Tau deposits in the brain. Tau-targeting therapies for AD have been unsuccessful in patients to date. Here we show that human polymerase δ-interacting protein 2 (PolDIP2) interacts with Tau. With a set of complementary methods, including thioflavin-T-based aggregation kinetic assays, Tau oligomer-specific dot-blot analysis, and single oligomer/fibril analysis by atomic force microscopy, we demonstrate that PolDIP2 inhibits Tau aggregation and amyloid fibril growth in vitro. The identification of PolDIP2 as a potential regulator of cellular Tau aggregation should be considered for future Tau-targeting therapeutics. |
Published |
Basel : MDPI |
Type |
Journal article |
Language |
English |
Publication date |
2021 |
CC license |
|