| Title |
Human polymerase δ-Interacting protein 2 (PolDIP2) inhibits the formation of human Tau oligomers and fibrils |
| Authors |
Kasho, Kazutoshi ; Krasauskas, Lukas ; Smirnovas, Vytautas ; Stojkovič, Gorazd ; Morozova-Roche, Ludmilla A ; Wanrooij, Sjoerd |
| DOI |
10.3390/ijms22115768 |
| Full Text |
|
| Is Part of |
International journal of molecular sciences.. Basel : MDPI. 2021, vol. 22, no. 11, art. no. 5768, p. [1-14].. eISSN 1422-0067 |
| Keywords [eng] |
PolDIP2 ; Tau ; amyloid |
| Abstract [eng] |
A central characteristic of Alzheimer's disease (AD) and other tauopathies is the accumulation of aggregated and misfolded Tau deposits in the brain. Tau-targeting therapies for AD have been unsuccessful in patients to date. Here we show that human polymerase δ-interacting protein 2 (PolDIP2) interacts with Tau. With a set of complementary methods, including thioflavin-T-based aggregation kinetic assays, Tau oligomer-specific dot-blot analysis, and single oligomer/fibril analysis by atomic force microscopy, we demonstrate that PolDIP2 inhibits Tau aggregation and amyloid fibril growth in vitro. The identification of PolDIP2 as a potential regulator of cellular Tau aggregation should be considered for future Tau-targeting therapeutics. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2021 |
| CC license |
|
