| Title |
Mikrodalelių, susidedančių iš DNR ir magnio pirofosfato, sintezė ir jų panaudojimas in vitro baltymų ekspresijai / |
| Translation of Title |
Synthesis of DNA and magnesium pyrophosphate particles and their use for protein expression in vitro. |
| Authors |
Galinis, Robertas |
| DOI |
10.15388/vu.thesis.193 |
| Full Text |
|
| Pages |
184 |
| Keywords [eng] |
microfluidics ; protein synthesis in vitro ; particles from magnesium pyrophosphate and DNA ; nonspecific DNA adsorption on the surfaces ; single DNA molecule amplification |
| Abstract [eng] |
In this work, a new method was described to apply magnesium pyrophosphate and DNA particles as a matrix for in vitro protein synthesis in microfluidic droplets. Single DNA molecules were encapsulated and amplified in 4 pl droplets. Optimized DNA amplification conditions were established to synthetize ~1 µm, magnesium pyrophosphate and DNA containing particles from single molecules. Also, reaction conditions were adjusted to reduce DNA molecule loss due to nonspecific adsorption on surfaces. Obtained particles were separated from reaction mixture. Finally, these particles were used to synthetize green fluorescent protein and β-galactosidase in microtiter plate and microfluidic droplets. |
| Dissertation Institution |
Vilniaus universitetas. |
| Type |
Doctoral thesis |
| Language |
Lithuanian |
| Publication date |
2021 |
