| Abstract [eng] |
Cells in different tissues have unique environments, which are important in maintaining tissue homeostasis. Even slight changes of this system can cause irreversible changes, induce the development of various diseases. Thus, in this work, we evaluated how mechanical properties of the growing surface impacts healthy and cancerous tissue cells. Gingiva mesenchymal stem cells (MSC) were chosen as representative of healthy tissue cells. These cells have gain a lot of attention in the field of regenerative medicine, due to wide range of their differentiation potential, immunomodulation functions and alleviation of ongoing oxidative stress (OS). For this reason, we evaluated how mechanical environment properties impacted MSC resistance to OS. We observed, that mechanical environment properties have influenced many processes of these cells, including their resistance to OS. The stiffer the surface was; the more resistant cells were. Breast cancer cell line MCF-7 was chosen as a model of cancerous tissue cells. Breast cancer is the most commonly diagnosed cancer and MCF-7 cell line represents the 70 % of all breast cancer cases phenotype. During the progression of cancer its environments gets stiffer and stiffer. Thus we investigated if mechanical properties of the surface have an impact to MCF-7 resistance to the chemotherapy drug doxorubicin. In this case, mechanical properties of the surface had no pronounce impact to MCF-7 sensitivity to doxorubicin, nevertheless, other tested properties of the cells depended to the stiffness of the surface, e.g. morphology, adhesion, proliferation, and even the expression and activation of signaling molecules. In addition to the mechanobiology research, a new method to evaluate cell number in 2D, 2.5D and 3D environment was developed. |
