| Title |
Vaccination with prion peptide-displaying polyomavirus-like particles prolongs incubation time in scrapie-infected mice / |
| Authors |
Eiden, Martin ; Gedvilaitė, Alma ; Leidel, Fabienne ; Ulrich, Rainer G ; Groschup, Martin H |
| DOI |
10.3390/v13050811 |
| Full Text |
|
| Is Part of |
Viruses.. Basel : MDPI. 2021, vol. 13, iss. 5, art. no. 811, p. [1-14].. eISSN 1999-4915 |
| Keywords [eng] |
active immunisation ; polyomavirus ; prion disease ; virus-like particles |
| Abstract [eng] |
Prion diseases like scrapie in sheep, bovine spongiform encephalopathy (BSE) in cattle or Creutzfeldt-Jakob disease (CJD) in humans are fatal neurodegenerative diseases characterized by the conformational conversion of the normal, mainly α-helical cellular prion protein (PrPC) into the abnormal β-sheet rich infectious isoform PrPSc. Various therapeutic or prophylactic approaches have been conducted, but no approved therapeutic treatment is available so far. Immunisation against prions is hampered by the self-tolerance to PrPC in mammalian species. One strategy to avoid this tolerance is presenting PrP variants in virus-like particles (VLPs). Therefore, we vaccinated C57/BL6 mice with nine prion peptide variants presented by hamster polyomavirus capsid protein VP1/VP2-derived VLPs. Mice were subsequently challenged intraperitoneally with the murine RML prion strain. Importantly, one group exhibited significantly increased mean survival time of 240 days post-inoculation compared with 202 days of the control group. These data show that immunisation with VLPs presenting PrP peptides may represent a promising strategy for an effective vaccination against transmissible spongiform encephalitis agents. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2021 |
| CC license |
|
