Title Dispersed conducting polymer nanocomposites with glucose oxidase and gold nanoparticles for the design of enzymatic glucose biosensors /
Authors German, Natalija ; Ramanavičienė, Almira ; Ramanavičius, Arūnas
DOI 10.3390/polym13132173
Full Text Download
Is Part of Polymers.. Basel : MDPI. 2021, vol. 13, iss. 13, art. no. 2173, p. 1-16.. ISSN 2073-4360
Keywords [eng] conducting polymers ; glucose biosensor ; glucose oxidase ; gold nanoparticles ; graphite rod electrode ; interference by ascorbic and uric acids ; polyaniline ; polypyrrole ; amperometry ; bioelectrochemistry
Abstract [eng] Biosensors for the determination of glucose concentration have a great significance in clinical diagnosis, and in the food and pharmaceutics industries. In this research, short-chain polyaniline (PANI) and polypyrrole (Ppy)-based nanocomposites with glucose oxidase (GOx) and 6 nm diameter AuNPs (AuNPs(6 nm)) were deposited on the graphite rod (GR) electrode followed by the immobilization of GOx. Optimal conditions for the modification of GR electrodes by conducting polymer-based nanocomposites and GOx were elaborated. The electrodes were investigated by cyclic voltammetry and constant potential amperometry in the presence of the redox mediator phenazine methosulfate (PMS). The improved enzymatic biosensors based on GR/PANI-AuNPs(6 nm)-GOx/GOx and GR/Ppy-AuNPs(6 nm)-GOx/GOx electrodes were characterized by high sensitivity (65.4 and 55.4 μA mM−1 cm−2), low limit of detection (0.070 and 0.071 mmol L−1), wide linear range (up to 16.5 mmol L−1), good repeatability (RSD 4.67 and 5.89%), and appropriate stability (half-life period (τ1/2) was 22 and 17 days, respectively). The excellent anti-interference ability to ascorbic and uric acids and successful practical application for glucose determination in serum samples was presented for GR/PANI-AuNPs(6 nm)-GOx/GOx electrode.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2021
CC license CC license description