| Abstract [eng] |
The dissertation describes experimental research in a relevant field of science - the development and improvement of molecularly imprinted polymer (MIP) sensitive to the most widely used herbicide in the world active ingredient – glyphosate. The electrically conductive polymer polypyrrole, which is easily obtained by electropolymerization, selected as a polymer matrix material. Although the electrochemical deposition of polypyrrole has been extensively studied, the mechanism of electrosynthesis is still not unambiguously confirmed. One of the aims of the dissertation is to investigate the peculiarities of polypyrrole layer formation on the gold surface and to find the most suitable electropolymerization conditions for MIP sensor electrosynthesis. In order to do so, concentration of polymerization solution and number of multicycle double-potential chronoamperometry steps during the polymerization were changed. After applying several research methods, the most suitable polypyrrole polymerization conditions for MIP sensor electrosynthesis was selected. Under the selected synthesis conditions, molecular glyphosate imprinted systems in polypyrrole matrix on bare gold and on the surface of self-assembled monolayer modified gold were developed and investigated. The paper describes the challenges that can be encountered in the formation of such systems and provides suggestions on how to address them. Glyphosate adsorption / desorption studies were also performed on the surfaces of the prepared systems and the resulting response was monitored and analyzed by SPR and EIS methods. The work description presents the physical characteristics of the investigated systems, determined using modern research methods, such as CV, EIS, SPR, AFM, etc. |
