| Title |
Functionalized protein nanotubes based on the bacteriophage vb_klem-rak2 tail sheath protein / |
| Authors |
Labutytė, Greta ; Povilonienė, Simona ; Šimoliūnas, Eugenijus ; Gabrielaitis, Dovydas ; Skapas, Martynas ; Noreika, Algirdas ; Meškys, Rolandas ; Časaitė, Vida |
| DOI |
10.3390/nano11113031 |
| Full Text |
|
| Is Part of |
Nanomaterials.. Basel : MDPI. 2021, vol. 11, no. 11, art. no. 3031, p. [1-14].. eISSN 2079-4991 |
| Keywords [eng] |
bacteriophage vB_KleM-RaK2 ; fluorescent nanoparticles ; functionalized nanoparticle ; green fluorescent protein ; MCherry ; nanotube ; self-assembly ; tail sheath protein ; YqfB |
| Abstract [eng] |
We report on the construction of functionalized nanotubes based on tail sheath protein 041 from vB_KleM-RaK2 bacteriophage. The truncated 041 protein (041∆200) was fused with fluorescent proteins GFP and mCherry or amidohydrolase YqfB. The generated chimeric proteins were successfully synthesized in E. coli BL21 (DE3) cells and self-assembled into tubular structures. We detected the fluorescence of the structures, which was confirmed by stimulated emission depletion microscopy. When 041∆200GFP and 041∆200mCherry were coexpressed in E. coli BL21 (DE3) cells, the formed nanotubes generated Förster resonance energy transfer, indicating that both fluorescent proteins assemble into a single nanotube. Chimeric 041∆200YqfB nanotubes possessed an enzymatic activity, which was confirmed by hydrolysis of N4-acetyl-2′-deoxycytidine. The enzymatic properties of 041∆200YqfB were similar to those of a free wild-type YqfB. Hence, we conclude that 041-based chimeric nanotubes have the potential for the development of delivery vehicles and targeted imaging and are applicable as scaffolds for biocatalysts. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2021 |
| CC license |
|
