| Title |
Scanning electrochemical microscopy for the investigation of biosensors / |
| Translation of Title |
Skenuojančios elektrocheminės mikroskopijos taikymas biologinių jutiklių tyrimuose. |
| Authors |
Kisieliūtė, Aura |
| DOI |
10.15388/vu.thesis.256 |
| Full Text |
|
| Pages |
120 |
| Keywords [eng] |
Scanning electrochemical microscopy ; biosensors ; redox ; immune sensors ; polypyrrole |
| Abstract [eng] |
Saccharomyces cerevisiae cells were electrochemically investigated in a double redox mediator system with varying lipophilic mediators and optimal parameters were found. The yeast cells were later modified with a conductive polymer - polypyrrole and multi walled carbon nanotubes for evaluating changes in the charge transfer. These modified cells were examined with atomic force microscopy, scanning electron microscopy, cyclic voltammetry and scanning electrochemical microscopy after the immobilization on a carbon felt electrode. The in-situ microorganism assisted polymerization with Aspergillus niger and Rhizoctania sp. cells was studied amperometrically by immobilizing them on a graphite rod and using it as a working electrode. The modification was also tested by immobilizing the cells on a Petri dish and evaluated by the Scanning electrochemical microscope. The other line of experiments involved the detection of the human growth hormone by electrochemical means. The antibodies against the human growth hormone were labelled with glucose oxidase which was detectable with the scanning electrochemical microscope in a solution containing glucose. The human growth hormone was detected in a non-competitive sandwich mode immunoassay with the glucose oxidase labelled human growth antibody in a solution containing glucose and potassium ferricyanide as the redox mediator in a substrate generation – tip collection scanning electrochemical microscopy mode. |
| Dissertation Institution |
Vilniaus universitetas. |
| Type |
Doctoral thesis |
| Language |
English |
| Publication date |
2021 |
