| Title |
Pantoea bacteriophage vB_PagS_MED16 - a siphovirus containing a 2'-deoxy-7-amido-7-deazaguanosine-modified DNA / |
| Authors |
Šimoliūnienė, Monika ; Žukauskienė, Emilija ; Truncaitė, Lidija ; Cui, Liang ; Hutinet, Geoffrey ; Kazlauskas, Darius ; Kaupinis, Algirdas ; Skapas, Martynas ; de Crécy-Lagard, Valérie ; Dedon, Peter ; Valius, Mindaugas ; Meškys, Rolandas ; Šimoliūnas, Eugenijus |
| DOI |
10.3390/ijms22147333 |
| Full Text |
|
| Is Part of |
International journal of molecular sciences.. Basel : MDPI. 2021, vol. 22, iss. 14, art. no. 7333, p. [1-21].. eISSN 1422-0067 |
| Keywords [eng] |
Pantoea agglomerans ; bacteriophage vB_PagS_AAS23 ; siphovirus ; 2'-deoxy-7-amido-7-deazaguanosine (dADG) |
| Abstract [eng] |
A novel siphovirus, vB_PagS_MED16 (MED16) was isolated in Lithuania using Pantoea agglomerans strain BSL for the phage propagation. The double-stranded DNA genome of MED16 (46,103 bp) contains 73 predicted open reading frames (ORFs) encoding proteins, but no tRNA. Our comparative sequence analysis revealed that 26 of these ORFs code for unique proteins that have no reliable identity when compared to database entries. Based on phylogenetic analysis, MED16 represents a new genus with siphovirus morphology. In total, 35 MED16 ORFs were given a putative functional annotation, including those coding for the proteins responsible for virion morphogenesis, phage–host interactions, and DNA metabolism. In addition, a gene encoding a preQ0 DNA deoxyribosyltransferase (DpdA) is present in the genome of MED16 and the LC–MS/MS analysis indicates 2'-deoxy-7-amido-7-deazaguanosine (dADG)-modified phage DNA, which, to our knowledge, has never been experimentally validated in genomes of Pantoea phages. Thus, the data presented in this study provide new information on Pantoea-infecting viruses and offer novel insights into the diversity of DNA modifications in bacteriophages. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2021 |
| CC license |
|
