| Title |
Bakteriofago vB_EcoS_NBD2 kilmės nanovamzdelių pritaikymas baltyminių fragmentų eksponavimui / |
| Translation of Title |
The application of bacteriophage vB_EcoS_NBD2 derived nanotubes for the presentation of protein fragments. |
| Authors |
Avižinienė, Aliona |
| DOI |
10.15388/vu.thesis.278 |
| Full Text |
|
| Pages |
168 |
| Keywords [eng] |
bacteriophage ; self-assembly ; nanotubes ; Acinetobacter baumannii, Saccharomyces cerevisiae |
| Abstract [eng] |
Viral nanoparticles can be used as immunogenic carriers for the presentation of foreign protein fragments and for the development of safe and effective vaccines. In this study, the nanotubes formed by the tail tube protein gp39 of bacteriophage vB_EcoS_NBD2 were characterized and their use as a carrier for the presentation of foreign protein fragments was evaluated. Saccharomyces cerevisiae-produced recombinant protein gp39 was able to self-assemble into nanotubes and the protein gp39 parts necessary for the nanotube formation were determined. The nanotubes were shown to be stable in various environmental conditions and immunogenic in mice. The protein gp39 was able to self-assemble into nanotubes when the foreign protein fragments were inserted into its C-terminus, but the nanotube formation depended on the physical and chemical properties of inserted protein fragments. Determined properties of gp39-tolerated protein fragments are important for successful chimeric nanotubes production. It was shown that nanotubes can be used as a carrier for the presentation of outer membrane protein OmpA fragments of opportunistic pathogen Acinetobacter baumannii. Since there is no vaccine against multi-drug resistant A. baumannii, chimeric nanotubes displaying OmpA protein fragments have a potential application to be used as an immunogenic carrier for vaccine development against A. baumannii. |
| Dissertation Institution |
Vilniaus universitetas. |
| Type |
Doctoral thesis |
| Language |
Lithuanian |
| Publication date |
2022 |
