| Title |
Hybrid bilayer membranes on metallurgical polished aluminum |
| Authors |
Sabirovas, Tomas ; Valiūnienė, Aušra ; Valinčius, Gintaras |
| DOI |
10.1038/s41598-021-89150-2 |
| Full Text |
|
| Is Part of |
Scientific reports.. Berlin : Nature research. 2021, vol. 11, iss. 1, art. no. 9648, p. [1-11].. ISSN 2045-2322 |
| Keywords [eng] |
bilayer membranes ; aluminum ; silanization ; phospholipids |
| Abstract [eng] |
In this work we describe the functionalization of metallurgically polished aluminum surfaces yielding biomimetic electrodes suitable for probing protein/phospholipid interactions. The functionalization involves two simple steps: silanization of the aluminum and subsequent fusion of multilamellar vesicles which leads to the formation of a hybrid bilayer lipid membrane (hBLM). The vesicle fusion was followed in real-time by fast Fourier transform electrochemical impedance spectroscopy (FFT EIS). The impedance-derived complex capacitance of the hBLMs was approximately 0.61 µF cm−2, a value typical for intact phospholipid bilayers. We found that the hBLMs can be readily disrupted if exposed to> 400 nM solutions of the pore-forming peptide melittin. However, the presence of cholesterol at 40% (mol) in hBLMs exhibited an inhibitory efect on the membrane-damaging capacity of the peptide. The melittin-membrane interaction was concentration dependent decreasing with concentration. The hBLMs on Al surface can be regenerated multiple times, retaining their dielectric and functional properties essentially intact. |
| Published |
Berlin : Nature research |
| Type |
Journal article |
| Language |
English |
| Publication date |
2021 |
| CC license |
|
