Title The butterfly effect: mild soil pollution with heavy metals elicits major biological consequences in cobalt-sensitized broad bean model plants /
Authors Šiukšta, Raimondas ; Pukenytė, Vėjūnė ; Kleizaitė, Violeta ; Bondzinskaitė, Skaistė ; Čėsnienė, Tatjana
DOI 10.3390/antiox11040793
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Is Part of Antioxidants.. Basel : MDPI. 2022, vol. 11, iss. 4, art. no. 793, p. [1-21].. eISSN 2076-3921
Keywords [eng] antioxidant response ; CDDP polymorphism ; Co pretreatment ; soil pollution ; transcript-based profiling ; Vicia faba
Abstract [eng] Among the heavy metals (HMs), only cobalt induces a polymorphic response in Vicia faba plants, manifesting as chlorophyll morphoses and a ‘break-through’ effect resulting in the elevated accumulation of other HMs, which makes Co-pretreated broad bean plants an attractive model for investigating soil pollution by HMs. In this study, Co-sensitized V. faba plants were used to evaluate the long-term effect of residual industrial pollution by examining biochemical (H2O2, ascorbic acid, malondialdehyde, free proline, flavonoid, polyphenols, chlorophylls, carotenoids, superoxide dismutase) and molecular (conserved DNA-derived polymorphism and transcript-derived polymorphic fragments) markers after long-term exposure. HM-polluted soil induced a significantly higher frequency of chlorophyll morphoses and lower levels of nonenzymatic antioxidants in Co-pretreated V. faba plants. Both molecular markers effectively differentiated plants from polluted and control soils into distinct clusters, showing that HMs in mildly polluted soil are capable of inducing changes in DNA coding regions. These findings illustrate that strong background abiotic stressors (pretreatment with Co) can aid investigations of mild stressors (slight levels of soil pollution) by complementing each other in antioxidant content reduction and induction of DNA changes.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2022
CC license CC license description