Title Adaptive response of Saccharomyces hosts to Totiviridae L-A dsRNA viruses Is achieved through intrinsically balanced action of targeted transcription factors /
Authors Ravoitytė, Bazilė ; Lukša, Juliana ; Wellinger, Ralf Erik ; Serva, Saulius ; Servienė, Elena
DOI 10.3390/jof8040381
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Is Part of Journal of fungi.. Basel : MDPI AG. 2022, vol. 8, iss. 4, art. no. 381, p. 1-24.. eISSN 2309-608X
Keywords [eng] Totiviridae ; dsRNA ; transcription ; yeast ; Saccharomyces cerevisiae ; Saccharomyces paradoxus
Abstract [eng] Totiviridae L-A virus is a widespread yeast dsRNA virus. The persistence of the L-A virus alone appears to be symptomless, but the concomitant presence of a satellite M virus provides a killer trait for the host cell. The presence of L-A dsRNA is common in laboratory, industrial, and wild yeasts, but little is known about the impact of the L-A virus on the host’s gene expression. In this work, based on high-throughput RNA sequencing data analysis, the impact of the L-A virus on whole-genome expression in three different Saccharomyces paradoxus and S. cerevisiae host strains was analyzed. In the presence of the L-A virus, moderate alterations in gene expression were detected, with the least impact on respiration-deficient cells. Remarkably, the transcriptional adaptation of essential genes was limited to genes involved in ribosome biogenesis. Transcriptional responses to L-A maintenance were, nevertheless, similar to those induced upon stress or nutrient availability. Based on these data, we further dissected yeast transcriptional regulators that, in turn, modulate the cellular L-A dsRNA levels. Our findings point to totivirus-driven fine-tuning of the transcriptional landscape in yeasts and uncover signaling pathways employed by dsRNA viruses to establish the stable, yet allegedly profitless, viral infection of fungi.
Published Basel : MDPI AG
Type Journal article
Language English
Publication date 2022
CC license CC license description