| Abstract [eng] |
This dissertation aimed to develop silver SERS substrates suitable for biomolecular analysis and to use them to investigate the applicability of mixed anchor monolayers for the construction of functional tethered bilayer lipid membrane (tBLM) models on silver. In this work, we developed a new fast and scalable manufacturing method of low-cost SERS substrates using inexpensive raw material – soda-lime glass. This method allows the rapid production of large quantities of uniform, repetitive nanostructured surfaces that produce substrates suitable for SERS measurements when coated with a silver coating. The optimal SERS signal amplification factor, which averages ~ 3x105 over the entire active area, has been achieved. Using these SERS substrates, we developed and characterised new different mixed self-assembled monolayers that could be applied to the construction of tBLM models on silver without causing corrosion and ensuring stability over time. In addition, using the EIS method, we have demonstrated that using our selected mixed self-assembled monolayers, we can generate functional and biologically relevant models of biological membranes on silver that could be applied to analytical biochemical studies. |
