Systematically higher Ki67 scores on core biopsy samples compared to corresponding resection specimen in breast cancer: a multi-operator and multi-institutional study /

Balazs Acs; Samuel C. Y Leung; Kelley M Kidwell; Indu Arun; Renaldas Augulis; Sunil S Badve; Yalai Bai; Anita L Bane; John M. S Bartlett; Jane Bayani; Gilbert Bigras; Annika Blank; Henk Buikema; Martin C Chang; Robin L Dietz; Andrew Dodson; Susan Fineberg; Cornelia M Focke; Dongxia Gao; Allen M Gown; Carolina Gutierrez; Johan Hartman; Zuzana Kos; Anne-Vibeke Lænkholm; Arvydas Laurinavičius; Richard M Levenson; Rustin Mahboubi-Ardakani; Mauro G Mastropasqua; Sharon Nofech-Mozes; C. Kent Osborne; Frédérique M Penault-Llorca; Tammy Piper; Mary Anne Quintayo; Tilman T Rau; Stefan Reinhard; Stephanie Robertson; Roberto Salgado; Tomoharu Sugie; Bert van der Vegt; Giuseppe Viale; Lila A Zabaglo; Daniel F Hayes; Mitch Dowsett; Torsten O Nielsen; David L Rimm; Lisa M McShane; Torsten Nielsen; Samuel Leung; Signe Borgquist; Angela Chan; Carsten Denkert; Anna Ehinger; Matthew Ellis; Margaret Flowers; Chad Galderisi; Abhi Gholap; Douglas J Hartman; Judith C Hugh; Anagha Jadhav; Elizabeth N Kornaga; Hans Kreipe; Richard Levenson; Mauro Mastropasqua; Takuya Moriya; Hongchao Pan; Liron Pantanowitz; Ernesta Paola Neri; Mei-Yin Polley; Jason Ruan; Takashi Sakatani; Lois Shepherd; Ian Smith; Joseph Sparano; Melanie Spears; Malini Srinivasan; Jane Starczynski; Austin Todd; Shakeel Virk; Yihong Wang; Hua Yang; Zhiwei Zhang; Inti Zlobec

doi:10.1038/s41379-022-01104-9

Title	Systematically higher Ki67 scores on core biopsy samples compared to corresponding resection specimen in breast cancer: a multi-operator and multi-institutional study /
Authors	Acs, Balazs ; Leung, Samuel C. Y ; Kidwell, Kelley M ; Arun, Indu ; Augulis, Renaldas ; Badve, Sunil S ; Bai, Yalai ; Bane, Anita L ; Bartlett, John M. S ; Bayani, Jane ; Bigras, Gilbert ; Blank, Annika ; Buikema, Henk ; Chang, Martin C ; Dietz, Robin L ; Dodson, Andrew ; Fineberg, Susan ; Focke, Cornelia M ; Gao, Dongxia ; Gown, Allen M ; Gutierrez, Carolina ; Hartman, Johan ; Kos, Zuzana ; Lænkholm, Anne-Vibeke ; Laurinavičius, Arvydas ; Levenson, Richard M ; Mahboubi-Ardakani, Rustin ; Mastropasqua, Mauro G ; Nofech-Mozes, Sharon ; Osborne, C. Kent ; Penault-Llorca, Frédérique M ; Piper, Tammy ; Quintayo, Mary Anne ; Rau, Tilman T ; Reinhard, Stefan ; Robertson, Stephanie ; Salgado, Roberto ; Sugie, Tomoharu ; van der Vegt, Bert ; Viale, Giuseppe ; Zabaglo, Lila A ; Hayes, Daniel F ; Dowsett, Mitch ; Nielsen, Torsten O ; Rimm, David L ; McShane, Lisa M ; Nielsen, Torsten ; Leung, Samuel ; Borgquist, Signe ; Chan, Angela ; Denkert, Carsten ; Ehinger, Anna ; Ellis, Matthew ; Flowers, Margaret ; Galderisi, Chad ; Gholap, Abhi ; Hartman, Douglas J ; Hugh, Judith C ; Jadhav, Anagha ; Kornaga, Elizabeth N ; Kreipe, Hans ; Levenson, Richard ; Mastropasqua, Mauro ; Moriya, Takuya ; Pan, Hongchao ; Pantanowitz, Liron ; Neri, Ernesta Paola ; Polley, Mei-Yin ; Ruan, Jason ; Sakatani, Takashi ; Shepherd, Lois ; Smith, Ian ; Sparano, Joseph ; Spears, Melanie ; Srinivasan, Malini ; Starczynski, Jane ; Todd, Austin ; Virk, Shakeel ; Wang, Yihong ; Yang, Hua ; Zhang, Zhiwei ; Zlobec, Inti
DOI	10.1038/s41379-022-01104-9
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Is Part of	Modern pathology.. London : Springer Science and Business Media LLC. 2022, vol. 35, p. 1362-1369.. ISSN 0893-3952. eISSN 1530-0285
Abstract [eng]	Ki67 has potential clinical importance in breast cancer but has yet to see broad acceptance due to inter-laboratory variability. Here we tested an open source and calibrated automated digital image analysis (DIA) platform to: (i) investigate the comparability of Ki67 measurement across corresponding core biopsy and resection specimen cases, and (ii) assess section to section differences in Ki67 scoring. Two sets of 60 previously stained slides containing 30 core-cut biopsy and 30 corresponding resection specimens from 30 estrogen receptor-positive breast cancer patients were sent to 17 participating labs for automated assessment of average Ki67 expression. The blocks were centrally cut and immunohistochemically (IHC) stained for Ki67 (MIB-1 antibody). The QuPath platform was used to evaluate tumoral Ki67 expression. Calibration of the DIA method was performed as in published studies. A guideline for building an automated Ki67 scoring algorithm was sent to participating labs. Very high correlation and no systematic error ( p = 0.08) was found between consecutive Ki67 IHC sections. Ki67 scores were higher for core biopsy slides compared to paired whole sections from resections ( p ≤ 0.001; median difference: 5.31%). The systematic discrepancy between core biopsy and corresponding whole sections was likely due to pre-analytical factors (tissue handling, fixation). Therefore, Ki67 IHC should be tested on core biopsy samples to best reflect the biological status of the tumor.
Published	London : Springer Science and Business Media LLC
Type	Journal article
Language	English
Publication date	2022
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„Systematically higher Ki67 scores on core biopsy samples compared to corresponding resection specimen in breast cancer: a multi-operator and multi-institutional study /“