| Title |
Towards an electrochemical immunosensor for the detection of antibodies against SARS-CoV-2 spike protein |
| Authors |
Liustrovaitė, Viktorija ; Drobysh, Maryia ; Ručinskienė, Alma ; Baradokė, Aušra ; Ramanavičienė, Almira ; Plikusienė, Ieva ; Samukaitė-Bubnienė, Urtė ; Viter, Roman ; Chen, Chien-Fu ; Ramanavičius, Arūnas |
| DOI |
10.1149/1945-7111/ac5d91 |
| Full Text |
|
| Is Part of |
Journal of the electrochemical society.. Pennington : The Electrochemical Society. 2022, vol. 169, iss. 3, art. no. 037523, p. 1-7.. ISSN 0013-4651. eISSN 1945-7111 |
| Keywords [eng] |
antigen-antibody complex. ; COVID-19 ; cyclic voltammetry ; electrochemical immunosensor ; electrochemical impedance spectroscopy ; SARS-CoV-2 ; self-assembled monolayer (SAM) |
| Abstract [eng] |
Herein we report the electrochemical system for the detection of specific antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins in blood serum patient samples after coronavirus disease 2019 (COVID-19). For this purpose, the recombinant SARS-CoV-2 spike protein (SCoV2-rS) was covalently immobilised on the surface of the gold electrode pre-modified with mixed self-assembled monolayer (SAMmix) consisting of 11-mercaptoundecanoic acid and 6-mercapto-1-hexanol. The affinity interaction of SCoV2-rS with specific antibodies against this protein (anti-rS) was detected using two electrochemical methods: cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The anti-rS was detected with a detection limit of 2.53 nM and 1.99 nM using CV and EIS methods, respectively. The developed electrochemical immunosensor is suitable for the confirmation of COVID-19 infection or immune response in humans after vaccination. |
| Published |
Pennington : The Electrochemical Society |
| Type |
Journal article |
| Language |
English |
| Publication date |
2022 |
| CC license |
|
