| Title |
FT-IR method limitations for β-glucan analysis |
| Authors |
Bikmurzin, Ruslan ; Bandzevičiūtė, Rimantė ; Maršalka, Arūnas ; Maneikis, Andrius ; Kalėdienė, Lilija |
| DOI |
10.3390/molecules27144616 |
| Full Text |
|
| Is Part of |
Molecules.. Basel : MDPI. 2022, vol. 27, iss.14, art. no. 4616, p. 1-14.. eISSN 1420-3049 |
| Keywords [eng] |
beta-glucans ; FT-IR ; solid-state ; NMR ; beta-1,3/1,6-linked glucans ; yeast glucans |
| Abstract [eng] |
β-glucans are known as biological response modifiers. However, different sources can result in structural differences and as a result differences in their biological activity. The hot water extraction method allows to obtain, high molecular weight β-glucans without altering their structure by using strong chemicals, such as alkalis or acids. Analysis of β-glucans by FT-IR and NMR spectroscopy in solid state is superior to analysis in solution as it allows researchers to study the preserved structure of the extracted polysaccharides. FT-IR spectroscopy was used in this study to make side-by-side comparison analysis of hot water extracted β-glucans from different yeast sources. NMR spectroscopy was used to confirm findings made by FT-IR spectroscopy. Extracted β-glucans exhibit characteristic structure of β-1,3/1,6-linked glucans with noticeable levels of proteins, possibly in a form of oligopeptides, chitin and other impurities. β-glucans obtained from C. guilliermondii, P. pastoris and S. pastorianus exhibited higher protein content. Differences in mannan, chitin and α-glucan content were also observed; however, the species-specific structure of obtained β-glucans could not be confirmed without additional studies. Structural analysis of high molecular weight β-glucans in solid state by FT-IR spectroscopy is difficult or limited due to band intensity changes and overlapping originating from different molecules. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2022 |
| CC license |
|
