| Title |
Electrochemical determination of interaction between SARS-CoV-2 spike protein and specific antibodies / |
| Authors |
Drobysh, Maryia ; Liustrovaitė, Viktorija ; Baradokė, Aušra ; Ručinskienė, Alma ; Ramanavičienė, Almira ; Ratautaitė, Vilma ; Viter, Roman ; Chen, Chien-Fu ; Plikusienė, Ieva ; Samukaitė-Bubnienė, Urtė ; Slibinskas, Rimantas ; Čiplys, Evaldas ; Simanavičius, Martynas ; Žvirblienė, Aurelija ; Kučinskaitė-Kodzė, Indrė ; Ramanavičius, Arūnas |
| DOI |
10.3390/ijms23126768 |
| Full Text |
|
| Is Part of |
International journal of molecular sciences.. Basel : MDPI AG. 2022, vol. 23, iss. 12, art. no. 6768, p. 1-10.. ISSN 1661-6596. eISSN 1422-0067 |
| Keywords [eng] |
antigen‐antibody complex ; COVID‐19 ; cyclic voltammetry (CV) ; electrochemical immunosensor ; electrochemical impedance spectroscopy (EIS) ; SARS‐CoV‐2 coronavirus ; self‐assembled monolayer (SAM) ; serological diagnosis ; specific antibodies ; spike proteins (rSpike) |
| Abstract [eng] |
The serologic diagnosis of coronavirus disease 2019 (COVID-19) and the evaluation of vaccination effectiveness are identified by the presence of antibodies specific to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this paper, we present the electrochemical-based biosensing technique for the detection of antibodies specific to the SARS-CoV-2 proteins. Recombinant SARS-CoV-2 spike proteins (rSpike) were immobilised on the surface of a gold electrode modified by a self-assembled monolayer (SAM). This modified electrode was used as a sensitive element for the detection of polyclonal mouse antibodies against the rSpike (anti-rSpike). Electrochemical impedance spectroscopy (EIS) was used to observe the formation of immunocomplexes while cyclic voltammetry (CV) was used for additional analysis of the surface modifications. It was revealed that the impedimetric method and the elaborate experimental conditions are appropriate for the further development of electrochemical biosensors for the serological diagnosis of COVID-19 and/or the confirmation of successful vaccination against SARS-CoV-2. |
| Published |
Basel : MDPI AG |
| Type |
Journal article |
| Language |
English |
| Publication date |
2022 |
| CC license |
|
