| Title |
Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines / |
| Authors |
Rakauskaitė, Rasa ; Urbanavičiūtė, Giedrė ; Rukšėnaitė, Audronė ; Liutkevičiūtė, Zita ; Juškėnas, Robertas ; Masevičius, Viktoras ; Klimašauskas, Saulius |
| DOI |
10.1039/c4cc07910h |
| Full Text |
|
| Is Part of |
Chemical communications.. Cambridge : The Royal Society of Chemistry. 2015, vol. 51, iss. 39, p. 8245-8248.. ISSN 1359-7345. eISSN 1364-548X |
| Keywords [eng] |
Selenocysteine ; selenoproteins ; protein dimerization |
| Abstract [eng] |
Selenocysteine is a valuable component of both natural selenoproteins and designer biocatalysts; however the availability of such proteins is hampered by technical limitations. Here we report the first general strategy for the production of selenoproteins via genetically-encoded incorporation of a synthetic photocaged selenocysteine residue in yeast cells, and provide examples of light-controlled protein dimerization and targeted covalent labeling in vitro. |
| Published |
Cambridge : The Royal Society of Chemistry |
| Type |
Journal article |
| Language |
English |
| Publication date |
2015 |
