Title |
Efficient disruption of Zebrafish genes using a Gal4-containing gene trap / |
Authors |
Balciuniene, Jorune ; Nagelberg, Danielle ; Walsh, Kathleen T ; Camerota, Diana ; Georlette, Daphné ; Biemar, Frédéric ; Bellipanni, Gianfranco ; Balčiūnas, Darius |
DOI |
10.1186/1471-2164-14-619 |
Full Text |
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Is Part of |
BMC genomics.. London : BMC. 2013, vol. 14, iss. 1, art. no. 619, p. [1-17].. ISSN 1471-2164 |
Keywords [eng] |
atp1a3a ; bbs7 ; Fleer ; Gal4 ; Gene trap ; Insertional mutagenesis ; nsfa ; Tol2 ; Zebrafish |
Abstract [eng] |
External development and optical transparency of embryos make zebrafish exceptionally suitable for in vivo insertional mutagenesis using fluorescent proteins to visualize expression patterns of mutated genes. Recently developed Gene Breaking Transposon (GBT) vectors greatly improve the fidelity and mutagenicity of transposon-based gene trap vectors. |
Published |
London : BMC |
Type |
Journal article |
Language |
English |
Publication date |
2013 |
CC license |
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