| Title |
Enhancement of glucose oxidase-based bioanode performance by comprising Spirulina platensis microalgae lysate / |
| Authors |
Žalnėravičius, Rokas ; Ramanavičius, Arūnas |
| DOI |
10.1149/1945-7111/ac7080 |
| Full Text |
|
| Is Part of |
Journal of the electrochemical society.. Pennington : The Electrochemical Society. 2022, vol. 169, iss. 5, art. no. 053510, p. 1-9.. ISSN 0013-4651. eISSN 1945-7111 |
| Keywords [eng] |
bioanode ; glucose oxidase (GOx) ; Glucose powered biofuel cell ; oxygen depletion ; Spirulina (Arthrospira) platensis |
| Abstract [eng] |
In this study, Spirulina platensis-based lysate was used as a biological redox mediator to design glucose oxidase (GOx) based biofuel cell bioanode. Chemically oxidized multi-walled carbon nanotubes (CNT) were deposited on carbon-electrode and were covered with eco-friendly algae cell-based lysate that facilitated the electron transfer and served as a biocompatible matrix for enzyme immobilization, which reduced the inactivation of GOx by CNT. The designed GC/PEI/CNT/S.p./GOx bioanode exhibited an open circuit potential (OCP) of-262 mV vs Ag/AgCl(3MKCl) in the presence of 12.5 mM of glucose. The maximum power output of the proposed bioanode was 21.8 times higher and reached 3.2 μW cm-2 at-51 mV vs Ag/AgCl(3MKCl) if the S. platensis cell lysate was used for bioanode design. The amperometric responses of GC/PEI/CNT/S.p./GOx bioelectrode towards the addition of glucose were linear at glucose concentrations ranging between 250 μM and 5 mM. These characteristics enable applying this bioanode as a part of biofuel cell and the electrode of an amperometric glucose biosensor, which response within 15 s, with a detection limit of 118 μM and a sensitivity of 15.09 μA mM-1 cm-2. |
| Published |
Pennington : The Electrochemical Society |
| Type |
Journal article |
| Language |
English |
| Publication date |
2022 |
| CC license |
|
