Title The significance of TNF-α and MMP-8 concentrations in non-invasively obtained amniotic fluid predicting fetal inflammatory response syndrome /
Authors Gulbinienė, Violeta ; Balčiūnienė, Greta ; Dumalakienė, Irena ; Vilienė, Rita ; Pilypienė, Ingrida ; Ramašauskaitė, Diana
DOI 10.1002/ijgo.14478
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Is Part of International journal of gynecology and obstetrics.. Hoboken : Wiley. 2023, vol. 160, no. 2, p. 476-482.. ISSN 0020-7292. eISSN 1879-3479
Keywords [eng] amniotic fluid ; biomarker ; fetal inflammatory response syndrome (FIRS) ; matrix metalloproteinase-8 (MMP-8) ; non-invasive method ; preterm birth ; preterm premature rupture of membranes (PPROM) ; tumor necrosis factor-α (TNF-α)
Abstract [eng] OBJECTIVE: To determine the significance of tumor necrosis factor-α (TNF-α) and matrix metalloproteinase-8 (MMP-8) in vaginally obtained amniotic fluid predicting fetal inflammatory response syndrome (FIRS) after preterm premature rupture of membranes (PPROM). METHODS: In this prospective case-control study, TNF-α and MMP-8 concentrations were evaluated in vaginally obtained amniotic fluid from women with PPROM at 22-34 weeks of pregnancy. Biomarkers' concentrations were determined using an enzyme-linked immunosorbent assay. Patients were divided into two groups: the FIRS group (cord blood interleukin-6 > 11 pg/ml or histological funisitis) and the non-FIRS group (without these findings). The data were analyzed using R package (R-4.0.5). RESULTS: The median TNF-α and MMP-8 concentrations in amniotic fluid from the 145 women included in the study were higher in the FIRS group than in the non-FIRS group. The area under the curve of TNF-α and MMP-8 was 0.77 and 0.75, respectively. The TNF-α concentration cut-off predicting FIRS was 89.20 pg/ml and was 170.76 pg/ml for MMP-8. In regression analysis, MMP-8 concentration was an independent predictor for FIRS. An MMP-8 concentration greater than 170 ng/ml and a TNF-α concentration greater than 89 pg/ml increased the odds of FIRS 7.62 and 14.92 times, respectively. CONCLUSIONS: MMP-8 and TNF-α concentrations in vaginally obtained amniotic fluid may be good predictors for FIRS after PPROM before 34 weeks of pregnancy. The non-invasive amniotic fluid analysis could be an alternative method to invasive amniocentesis.
Published Hoboken : Wiley
Type Journal article
Language English
Publication date 2023
CC license CC license description