| Title |
Association of ScV-LA virus with host protein metabolism determined by proteomics analysis and cognate RNA sequencing / |
| Authors |
Lukša, Juliana ; Celitan, Enrika ; Servienė, Elena ; Serva, Saulius |
| DOI |
10.3390/v14112345 |
| Full Text |
|
| Is Part of |
Viruses: Omics of virus-host interactions.. Basel : MDPI. 2022, vol. 14, iss. 11, art. no. 2345, p. [1-19].. eISSN 1999-4915 |
| Keywords [eng] |
Saccharomyces cerevisiae ; yeast virus ; Totiviridae ; dsRNA ; proteomics ; transcriptomics |
| Abstract [eng] |
Saccharomyces yeasts are highly dispersed in the environment and microbiota of higher organisms. The yeast killing phenotype, encoded by the viral system, was discovered to be a significant property for host survival. Minor alterations in transcription patterns underpin the reciprocal relationship between LA and M viruses and their hosts, suggesting the fine-tuning of the transcriptional landscape. To uncover the principal targets of both viruses, we performed proteomics analysis of virus-enriched subsets of host proteins in virus type-specific manner. The essential pathways of protein metabolism–from biosynthesis and folding to degradation–were found substantially enriched in virus-linked subsets. The fractionation of viruses allowed separation of virus-linked host RNAs, investigated by high-content RNA sequencing. Ribosomal RNA was found to be inherently associated with LA-lus virus, along with other RNAs essential for ribosome biogenesis. This study provides a unique portrayal of yeast virions through the characterization of the associated proteome and cognate RNAs, and offers a background for understanding ScV-LA viral infection persistency. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2022 |
| CC license |
|
