| Abstract [eng] |
We aim to introduce and describe several glucose biosensor and glucose-powered biofuel cell technologies. We describe a bio electrochemical application of electrochemically generated hole-transporting (p-type) polymeric semiconductors (HTPS), which are based on carbazole derivatives and triphelamine as direct charge transfer glucose sensors. Electrodes were electrochemically modified with a layer of HTPS and a monolayer of covalently immobilized glucose oxidase (GOx). The HTPS/GOx-based electrodes were investigated for an evaluation of direct hole-transfer between the enzyme and electrode at a bio-electrochemically relevant potential. Enzymatic biofuel cell (EBFC) systems were explored with different redox mediator materials used to evaluate their applicability. Redox mediators of different natures have been selected to provide a deeper understanding of the charge transfer system and their effects on the characteristics of biofuel cells. Cytochrome c, Chlorophyll a, and supernatant of ultrasonically disrupted algae Chlorella vulgaris cells were examined as potential redox mediators. We have assessed the applicability of the microorganism Rhizobium anhuiense as a catalyst suitable for the design of microbial fuel cells (MFBC). The Rhizobium anhuiense bacteria were cultivated in a high glucose environment and to improve the charge transfer, several redox mediators were tested, namely menadione, riboflavin, and 9,10-phenanthrenequinone (PQ). |
