| Title |
Determination of steroid hormones in grey seal (Halichoerus grypus) blood plasma using convergence chromatography tandem mass spectrometry / |
| Authors |
Lea Sait, Shannen Thora ; Survilienė, Vaida ; Jüssi, Mart ; Gonzalez, Susana V ; Ciesielski, Tomasz Maciej ; Jenssen, Bjørn Munro ; Asimakopoulos, Alexandros G |
| DOI |
10.1016/j.talanta.2022.124109 |
| Full Text |
|
| Is Part of |
Talanta.. Amsterdam : Elsevier B.V.. 2023, vol. 254, art. no.124109, p. [1-13].. ISSN 0039-9140. eISSN 1873-3573 |
| Keywords [eng] |
Convergence chromatography ; Pinnipeds ; Marine mammals ; Cortisol |
| Abstract [eng] |
A hybrid solid phase extraction (HybridSPE) protocol tailored to ultra-performance convergence chromatography tandem mass spectrometry (UPC2-MS/MS) was developed for the determination of 19 steroid hormones in grey seal (Halichoerus grypus) blood plasma. In this study, the protocol demonstrated acceptable absolute recoveries ranging from 33 to 90%. The chromatographic separation was carried out using a gradient elution program with a total run time of 5 min. The method repeatability ranged from 1.9% to 24% for most target analytes and the method limits of quantification (mLOQs) ranged from 0.03 to 1.67 ng/mL. A total of 9 plasma samples were analysed to demonstrate the applicability of the developed method in blood plasma from a wildlife species. A total of 13 steroid hormones were quantified in grey seal pup plasma. The most prevalent steroids: cortisol, cortisone, corticosterone, 11-deoxycortisol, progesterone and 17α-hydroxyprogesterone were detected at concentrations in the range of 9.09–40.1, 7.10–24.2, 0.742–10.7, 1.06–5.72, 0.38–4.38 and <mLOQ - 1.05 ng/mL, respectively. To our knowledge, this is the first study to determine steroid hormones in the plasma of pinnipeds using convergence chromatography. |
| Published |
Amsterdam : Elsevier B.V |
| Type |
Journal article |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
