| Title |
Monoclonal antibodies against the newly identified allergen β-enolase from common carp (Cyprinus carpio) / |
| Authors |
Sližienė, Aistė ; Plečkaitytė, Milda ; Zaveckas, Mindaugas ; Juškaitė, Karolina ; Rudokas, Vytautas ; Žvirblis, Gintautas ; Žvirblienė, Aurelija |
| DOI |
10.1080/09540105.2022.2028741 |
| Full Text |
|
| Is Part of |
Food and agricultural immunology.. Abingdon : Taylor and Francis Ltd.. 2022, vol. 33, iss. 1, p. 129-149.. ISSN 0954-0105. eISSN 1465-3443 |
| Keywords [eng] |
allergen extracts ; common carp ; cross-reactivity ; monoclonal antibodies ; β-enolase |
| Abstract [eng] |
β-enolase is a heat-labile fish allergen identified in different fish species. In this study, β-enolase gene was cloned from common carp muscle tissue and the target protein was expressed as a fusion with maltose binding protein (MBP-Eno) in E. coli. Recombinant MBP-Eno was reactive with blood serum specimens of fish-allergic patients, confirming that β-enolase is a newly identified allergen of common carp. Two monoclonal antibodies (MAbs) against recombinant β-enolase were generated that showed cross-reactivity with β-enolases of other organisms. Both antibodies recognized β-enolases from other fish species extracts, while MAb 6E4 showed a broad reactivity with pork, chicken, yeast, and E. coli samples. Epitope mapping using MBP-Eno variants revealed that β-enolase region comprising amino acids 623–698 presumably includes MAb 6E4 epitope. The generated broadly reactive MAb 6E4 directed against a conserved epitope of β-enolases may represent a valuable tool for the characterization of allergen extracts and fish allergy diagnostics. |
| Published |
Abingdon : Taylor and Francis Ltd |
| Type |
Journal article |
| Language |
English |
| Publication date |
2022 |
| CC license |
|
