| Title |
Diverse virus-encoded CRISPR-Cas systems include streamlined genome editors / |
| Authors |
Al-Shayeb, Basem ; Skopintsev, Petr ; Soczek, Katarzyna M ; Stahl, Elizabeth C ; Li, Zheng ; Groover, Evan ; Smock, Dylan ; Eggers, Amy R ; Pausch, Patrick ; Cress, Brady F ; Huang, Carolyn J ; Staskawicz, Brian ; Savage, David F ; Jacobsen, Steven E ; Banfield, Jillian F ; Doudna, Jennifer A |
| DOI |
10.1016/j.cell.2022.10.020 |
| Full Text |
|
| Is Part of |
Cell.. Cambridge, MA : Cell Press. 2022, vol. 185, iss. 24, p. 4574-4586, e1-e16.. ISSN 0092-8674. eISSN 1097-4172 |
| Keywords [eng] |
CRISPR ; CRISPR-Cas ; phage ; metagenomics ; genome editing ; genome editor ; anti-viral ; structure ; enzyme ; tool |
| Abstract [eng] |
CRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find that CRISPR systems are also encoded in diverse bacteriophages, where they occur as divergent and hypercompact anti-viral systems. Bacteriophage-encoded CRISPR systems belong to all six known CRISPR-Cas types, though some lack crucial components, suggesting alternate functional roles or host complementation. We describe multiple new Cas9-like proteins and 44 families related to type V CRISPR-Cas systems, including the Casλ RNA-guided nuclease family. Among the most divergent of the new enzymes identified, Casλ recognizes double-stranded DNA using a uniquely structured CRISPR RNA (crRNA). The Casλ-RNA-DNA structure determined by cryoelectron microscopy reveals a compact bilobed architecture capable of inducing genome editing in mammalian, Arabidopsis, and hexaploid wheat cells. These findings reveal a new source of CRISPR-Cas enzymes in phages and highlight their value as genome editors in plant and human cells. |
| Published |
Cambridge, MA : Cell Press |
| Type |
Journal article |
| Language |
English |
| Publication date |
2022 |
| CC license |
|
