| Title |
Protocol to dissociate, process, and analyze the human lung tissue using single-cell RNA-seq |
| Authors |
Quintanal-Villalonga, Álvaro ; Chan, Joseph M ; Masilionis, Ignas ; Gao, Vianne Ran ; Xie, Yubin ; Allaj, Viola ; Chow, Andrew ; Poirier, John T ; Pe'er, Dana ; Rudin, Charles M ; Mažutis, Linas |
| DOI |
10.1016/j.xpro.2022.101776 |
| Full Text |
|
| Is Part of |
STAR protocols.. New York : Cell Press. 2022, vol. 3, no. 4, art. no. 101776, p. [1-25].. eISSN 2666-1667 |
| Keywords [eng] |
cancer ; cell isolation ; flow cytometry/mass cytometry ; molecular biology ; RNAseq ; sequence analysis ; sequencing ; single cell |
| Abstract [eng] |
We report a protocol for obtaining high-quality single-cell transcriptomics data from human lung biospecimens acquired from core needle biopsies, fine-needle aspirates, surgical resection, and pleural effusions. The protocol relies upon the brief mechanical and enzymatic disruption of tissue, enrichment of live cells by fluorescence-activated cell sorting (FACS), and droplet-based single-cell RNA sequencing (scRNA-seq). The protocol also details a procedure for analyzing the scRNA-seq data. For complete details on the use and execution of this protocol, please refer to Chan et al. (2021). |
| Published |
New York : Cell Press |
| Type |
Journal article |
| Language |
English |
| Publication date |
2022 |
| CC license |
|
