| Title |
Electrochemical biosensor for the determination of specific antibodies against SARS-CoV-2 spike protein / |
| Authors |
Žukauskas, Šarūnas ; Ručinskienė, Alma ; Ratautaitė, Vilma ; Ramanavičienė, Almira ; Pilvenytė, Greta ; Bechelany, Mikhael ; Ramanavičius, Arūnas |
| DOI |
10.3390/ijms24010718 |
| Full Text |
|
| Is Part of |
International journal of molecular sciences.. Basel : MDPI AG. 2023, vol. 24, iss. 1, art. no. 718, p. 1-14.. eISSN 1422-0067 |
| Keywords [eng] |
coronavirus disease 2019 (COVID-19) ; SARS-CoV-2 virus proteins ; specific antibodies ; immune complex ; electrochemical biosensor ; immunosensor ; self-assembled monolayer (SAM) ; polycrystalline gold electrode ; cyclic voltammetry ; differential pulse voltammetry ; potential pulsed amperometry |
| Abstract [eng] |
In this article, we report the development of an electrochemical biosensor for the determination of the SARS-CoV-2 spike protein (rS). A gold disc electrode was electrochemically modified to form the nanocrystalline gold structure on the surface. Then, it was further altered by a self-assembling monolayer based on a mixture of two alkane thiols: 11-mercaptoundecanoic acid (11-MUA) and 6-mercapto-1-hexanol (6-MCOH) (SAMmix). After activating carboxyl groups using a N-(3-dimethylaminopropyl)-N’-ethyl-carbodiimide hydrochloride and N-hydroxysuccinimide mixture, the rS protein was covalently immobilized on the top of the SAMmix. This electrode was used to design an electrochemical sensor suitable for determining antibodies against the SARS-CoV-2 rS protein (anti-rS). We assessed the association between the immobilized rS protein and the anti-rS antibody present in the blood serum of a SARS-CoV-2 infected person using three electrochemical methods: cyclic voltammetry, differential pulse voltammetry, and potential pulsed amperometry. The results demonstrated that differential pulse voltammetry and potential pulsed amperometry measurements displayed similar sensitivity. In contrast, the measurements performed by cyclic voltammetry suggest that this method is the most sensitive out of the three methods applied in this research. |
| Published |
Basel : MDPI AG |
| Type |
Journal article |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
