Title Establishing reference intervals for von Willebrand factor multimers /
Authors Pikta, Marika ; Vasse, Marc ; Smock, Kristi J ; Moser, Karen A ; van Dievoet, Marie-Astrid ; Lejniece, Sandra ; Szanto, Timea ; Bautista, Hector ; Nouadje, George ; Banys, Valdas
DOI 10.5937/JOMB0-31941
Full Text Download
Is Part of Journal of medical biochemistry.. Society of Medical Biochemists of Serbia. 2022, vol. 41, no. 1, p. 115-121.. ISSN 1452-8258. eISSN 1452-8266
Keywords [eng] quantitative analysis ; reference intervals ; von Willebrand factor ; von Willebrand factor multimers
Abstract [eng] Background: von Willebrand factor (VWF) multimers (VWF:MM) methodologies are technically difficult, laborious, time consuming, non-standardized and results vary between laboratories. A new semi automated VWF:MM assay is available for routine use (Sebia). Due to lack of reference values for VWF:MM fractions, results interpretation can be challenging in some cases. The aim of this study was to determine reference intervals for low molecular weight (LMWM), intermediate molecular weight (IMWM) and high molecular weight (HMWM) multimers. Methods: By the international cooperation initiated between 4 countries (Estonia, Latvia, France, and USA) 131 samples of relatively healthy individuals were analyzed for VWF:MM (in total 51 males and 80 non-pregnant females aged 17-69 years). Reference intervals were calculated according to CLSI C28-A3 standard. Results: The proposed reference intervals for VWF:MM were calculated for LMWM 10.4-22.5%, IMWM 22.6-37.6%, HMWM 45.6-66.6%. Age related differences were seen in IMWM and HMWM (p<0.001 and 0.038). There was no gender related difference observed. Geographically LMWM results of France were different from the other regions (p<0.05). Conclusions: Quantification of VWF:MM fractions, in addition to qualitative assessment of VWF:MM patterns, has the potential to aid in differential diagnosis of von Willebrand disease (VWD) subtypes. The reference values calculated in this study can be used in future research to establish clinical decision limits.
Published Society of Medical Biochemists of Serbia
Type Journal article
Language English
Publication date 2022
CC license CC license description