Title Characterization of a panel of cross-reactive hantavirus nucleocapsid protein-specific monoclonal antibodies /
Authors Avižinienė, Aliona ; Kučinskaitė-Kodzė, Indrė ; Petraitytė-Burneikienė, Rasa ; Žvirblienė, Aurelija ; Mertens, Marc L ; Schmidt, Sabrina ; Schlegel, Mathias ; Lattwein, Erik ; Koellner, Bernd ; Ulrich, Rainer G
DOI 10.3390/v15020532
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Is Part of Viruses.. Basel : MDPI. 2023, vol. 15, iss. 2, art. no. 532, p. [1-18].. eISSN 1999-4915
Keywords [eng] cross-reactivity ; epitope mapping ; hantavirus ; monoclonal antibody ; nucleocapsid protein ; Saccharomyces cerevisiae
Abstract [eng] Hantaviruses are emerging pathogens with a worldwide distribution that can cause life-threatening diseases in humans. Monoclonal antibodies (MAbs) against hantavirus nucleocapsid (N) proteins are important tools in virus diagnostics, epidemiological studies and basic research studies on virus replication and pathogenesis. Here, we extend the collection of previously generated MAbs raised against a segment of Puumala orthohantavirus (PUUV) N protein harbored on virus-like particles (VLPs) and MAbs against N proteins of Sin Nombre orthohantavirus/Andes orthohantavirus by generating nine novel MAbs against N proteins of Dobrava-Belgrade orthohantavirus (DOBV), Tula orthohantavirus (TULV), Thottapalayam thottimvirus (TPMV) and PUUV. In order to have a wide collection of well-described hantavirus-specific MAbs, the cross-reactivity of novel and previously generated MAbs was determined against N proteins of 15 rodent- and shrew-borne hantaviruses by different immunological methods. We found that all MAbs, excluding TPMV-specific MAbs, demonstrated different cross-reactivity patterns with N proteins of hantaviruses and recognized native viral antigens in infected mammalian cells. This well-characterized collection of cross-reactive hantavirus-specific MAbs has a potential application in various fields of hantavirus research, diagnostics and therapy.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2023
CC license CC license description