| Title |
Uterine cavity lavage mutation analysis in Lithuanian ovarian cancer patients / |
| Authors |
Žilovič, Diana ; Vaicekauskaitė, Ieva ; Čiurlienė, Rūta ; Sabaliauskaitė, Rasa ; Jarmalaitė, Sonata |
| DOI |
10.3390/cancers15030868 |
| Full Text |
|
| Is Part of |
Cancers.. Basel : MDPI. 2023, vol. 15, iss. 3, art. no. 868, p. [1-13].. eISSN 2072-6694 |
| Keywords [eng] |
uterine lavage ; ovarian cancer ; liquid biopsy ; TP53 |
| Abstract [eng] |
Background: Type II ovarian cancer (OC) is generally diagnosed at an advanced stage, translating into a poor survival rate. Current screening methods for OC have failed to demonstrate a reduction in mortality. The uterine lavage technique has been used to detect tumor-specific TP53 mutations from cells presumably shed from high-grade serous ovarian cancer (HGSOC). We aimed to pilot whether the detection of TP53 mutation in uterine cavity lavage can be used as a diagnostic method for HGSOC using an expanded gene panel. Methods: In this study 90, uterine lavage and 46 paired biopsy samples were analyzed using next-generation sequencing (NGS) targeting TP53 as well as five additional OC-related genes: BRCA1, BRCA2, PI3KCA, PTEN, and KRAS. Results: Uterine lavage was successfully applied to all patients, and 56 mutations were detected overall. TP53 mutations were detected in 27% (10/37) of cases of type HGSOC; BRCA1 and BRCA2 mutations were also frequent in this group (46%; 17/37). Overall concordance between tissue and liquid biopsy samples was 65.2%. Conclusion: Uterine lavage TP53 mutations in combination with other biomarkers could be a useful tool for the detection of lowly invasive HGSOC. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
