Title Electrochemical immunosensor for the determination of antibodies against prostate-specific antigen based on ZnO nanostructures /
Authors Liustrovaitė, Viktorija ; Karoblis, Dovydas ; Brasiūnas, Benediktas ; Popov, Anton ; Katelnikovas, Artūras ; Kareiva, Aivaras ; Ramanavičius, Arūnas ; Viter, Roman ; Giardi, Maria Teresa ; Erts, Donats ; Ramanavičienė, Almira
DOI 10.3390/ijms24065803
Full Text Download
Is Part of International journal of molecular sciences.. Basel : MDPI. 2023, vol. 24, iss. 6, art. no. 5803, p. [1-14].. ISSN 1661-6596. eISSN 1422-0067
Keywords [eng] antigen–antibody complex ; cyclic voltammetry ; differential pulse voltammetry ; electrochemical immunosensor ; one-dimensional nanostructures ; zinc oxide nanostructures
Abstract [eng] In this study, ZnO nanostructures with different types of morphologies and particle sizes were evaluated and applied for the development of an immunosensor. The first material was composed of spherical, polydisperse nanostructures with a particle size in the range of 10–160 nm. The second was made up of more compact rod-like spherical nanostructures with the diameter of these rods in the range of 50–400 nm, and approximately 98% of the particles were in the range of 20–70 nm. The last sample of ZnO was made up of rod-shaped particles with a diameter of 10–80 nm. These ZnO nanostructures were mixed with Nafion solution and drop-casted onto screen-printed carbon electrodes (SPCE), followed by a further immobilization of the prostate-specific antigen (PSA). The affinity interaction of PSA with monoclonal antibodies against PSA (anti-PSA) was evaluated using the differential pulse voltammetry technique. The limit of detection and limit of quantification of anti-PSA were determined as 1.35 nM and 4.08 nM for compact rod-shaped spherical ZnO nanostructures, and 2.36 nM and 7.15 nM for rod-shaped ZnO nanostructures, respectively.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2023
CC license CC license description