| Title |
SARS-CoV-2 RT-qPCR Ct values in saliva and nasopharyngeal swab samples for disease severity prediction / |
| Authors |
Šnipaitienė, Kristina ; Zablockienė, Birutė ; Sabaliauskaitė, Rasa ; Žukauskaitė, Kristina ; Matulytė, Elžbieta ; Smalinskaitė, Tautvilė ; Paulauskas, Mindaugas ; Zablockis, Rolandas ; Lopeta, Mantvydas ; Gagilas, Julius ; Pūrienė, Alina ; Jančorienė, Ligita ; Jarmalaitė, Sonata |
| DOI |
10.1080/20002297.2023.2213106 |
| Full Text |
|
| Is Part of |
Journal of oral microbiology.. Abingdon : Taylor & Francis. 2023, vol. 15, no. 1, art. no. 2213106, p. [1-12].. eISSN 2000-2297 |
| Keywords [eng] |
SARS-CoV-2 ; COVID-19 ; severity ; saliva ; RT-qPCR ; Ct values ; viral dynamica |
| Abstract [eng] |
Background: Comparison of clinical value of RT-qPCR-based SARS-CoV-2 tests performed on saliva samples (SSs) and nasopharyngeal swab samples (NPSs) for prediction of the COVID-19 disease severity.Methods: Three paired SSs and NPSs collected every 3 days from 100 hospitalised COVID-19 patients during 2020 Jul-2021 Jan were tested by RT-qPCR for the original SARS-CoV-2 virus and compared to 150 healthy controls. Cases were divided into mild+moderate (Cohort I, N = 47) and severe disease (Cohort II, N = 53) cohorts and compared.Results: SARS-CoV-2 was detected in 65% (91/140) vs. 53% (82/156) of NPSs and 49% (68/ 139) vs. 48% (75/157) of SSs collected from Cohort I and II, respectively, resulting in the total respective detection rates of 58% (173/296) vs. 48% (143/296) (P = 0.017). Ct values of SSs were lower than those of NPSs (mean Ct = 28.01 vs. 30.07, P = 0.002). Although Ct values of the first SSs were significantly lower in Cohort I than in Cohort II (P = 0.04), it became negative earlier (mean 11.7 vs. 14.8 days, P = 0.005). Multivariate Cox proportional hazards regression analysis showed that Ct value ≤30 from SSs was the independent predictor for severe COVID- 19 (HR = 10.06, 95% CI: 1.84–55.14, P = 0.008 Conclusion: Salivary RT-qPCR testing is suitable for SARS-CoV-2 infection control, while imple measurement of Ct values can assist in prediction of COVID-19 severity. |
| Published |
Abingdon : Taylor & Francis |
| Type |
Journal article |
| Language |
English |
| Publication date |
2023 |
| CC license |
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