| Title |
Exploring two-photon optogenetics beyond 1100 nm for specific and effective all-optical physiology |
| Authors |
Fu, Ting ; Arnoux, Isabelle ; Doering, Jan ; Backhaus, Hendrik ; Watari, Hirofumi ; Stasevičius, Ignas ; Fan, Wei ; Stroh, Albrecht |
| DOI |
10.1016/j.isci.2021.102184 |
| Full Text |
|
| Is Part of |
Iscience.. Cambridge : Elsevier. 2021, vol. 24, iss. 3, art. no. 102184, p. [1-31].. eISSN 2589-0042 |
| Abstract [eng] |
Two-photon (2-P) all-optical approaches combine in vivo 2-P calcium imaging and 2-P optogenetic modulations. Here, firstly, we combined in vivo juxtacellular recordings and GCaMP6f-based 2-P calcium imaging in mouse visual cortex to tune our detection algorithm towards a 100% specific identification of action potential-related calcium transients. Secondly, we minimized photostimulation artifacts by using extended-wavelength-spectrum laser sources for optogenetic stimulation. We achieved artifact-free all-optical experiments performing optogenetic stimulation from 1100 nm to 1300 nm. Thirdly, we determined the spectral range for maximizing efficacy until 1300 nm. The rate of evoked transients in GCaMP6f/C1V1-co-expressing cortical neurons peaked already at 1100 nm. By refining spike detection and defining 1100 nm as the optimal wavelength for artifact-free and effective GCaMP6f/C1V1-based all-optical physiology, we increased the translational value of these approaches, e.g., for the development of network-based therapies. |
| Published |
Cambridge : Elsevier |
| Type |
Journal article |
| Language |
English |
| Publication date |
2021 |
| CC license |
|
