| Title |
Novel leaderless bacteriocin geobacillin 6 from thermophilic bacterium Parageobacillus thermoglucosidasius / |
| Authors |
Koniuchovaitė, Ana ; Petkevičiutė, Akvilė ; Bernotaitė, Emilija ; Gricajeva, Alisa ; Gegeckas, Audrius ; Kalėdienė, Lilija ; Kaunietis, Arnoldas |
| DOI |
10.3389/fmicb.2023.1207367.s002 |
| Full Text |
|
| Is Part of |
Frontiers in microbiology.. Lausanne : Frontiers Media SA. 2023, vol. 14, art. no. 1207367, p. [1-12].. eISSN 1664-302X |
| Keywords [eng] |
leaderless bacteriocin ; thermophilic bacteria ; geobacillin ; Parageobacillus ; heterologous expression. |
| Abstract [eng] |
Bacterial resistance to conventional antibiotics has urged us to develop alternative strategies against bacterial pathogens. Moreover, a demand for food products containing no chemical preservatives has led us to search for new alternative technologies for food preservation. Bacteriocins – ribosomally synthesized antimicrobial peptides – have been proposed as a new alternative to conventional antibiotics or chemicals for food preservation. This study describes biosynthesis and characterization of a novel leaderless bacteriocin, geobacillin 6, which was identified in the thermophilic bacterium Parageobacillus thermoglucosidasius. Its amino acid sequence shows low similarity to other bacteriocins and it is the first leaderless-type bacteriocin identified in thermophilic bacteria. Based on structure assessment, the bacteriocin forms a multi-helix bundle. Geobacillin 6 exhibits a relatively narrow antimicrobial spectrum, it is active in the μM range and against Gram-positive bacteria, mostly thermophilic species closely related to the producer strain. Bacteriocin demonstrates stability over pH 3–11 and is highly thermostable, retaining 100% of its activity after incubation at 95°C for 6 h. Geobacillin 6 has potential in the food industry and biotechnological processes where contamination with thermophilic bacteria is undesirable. |
| Published |
Lausanne : Frontiers Media SA |
| Type |
Journal article |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
