Title Stimulation of chondrocyte and bone marrow mesenchymal stem cell chondrogenic response by polypyrrole and polypyrrole/gold nanoparticles /
Authors Uzielienė, Ilona ; Popov, Anton ; Lisytė, Viktorija ; Kugaudaitė, Gabija ; Bialaglovytė, Paulina ; Vaičiulevičiūtė, Raminta ; Kvederas, Giedrius ; Bernotienė, Eiva ; Ramanavičienė, Almira
DOI 10.3390/polym15112571
Full Text Download
Is Part of Polymers: Progress in the Formation of Polymer-Based Nanomaterials for New Applications.. Basel : MDPI. 2023, vol. 15, iss. 11, art. no. 2571, p. 1-15.. eISSN 2073-4360
Keywords [eng] bone marrow mesenchymal stem cells ; chondrocytes ; chondrogenic differentiation ; polypyrrole/gold nanoparticles
Abstract [eng] Bone marrow mesenchymal stem cells (BMMSCs) possess a strong ability to differentiate into the chondrogenic lineage, which is important for cartilage regeneration. External stimuli, such as electrical stimulation (ES), are frequently studied for chondrogenic differentiation of BMMSCs; however, the application of conductive polymers such as polypyrrole (Ppy), has never been used for stimulating BMMSCs chondrogenesis in vitro before. Thus, the aim of this study was to evaluate the chondrogenic potential of human BMMSCs after stimulation with Ppy nanoparticles (Ppy NPs) and compare them to cartilage-derived chondrocytes. In this study, we tested Ppy NPs without and with 13 nm gold NPs (Ppy/Au) for BMMSCs and chondrocyte proliferation, viability, and chondrogenic differentiation for 21 days, without the use of ES. The results demonstrated significantly higher amounts of cartilage oligomeric matrix protein (COMP) in BMMSCs stimulated with Ppy and Ppy/Au NPs, as compared to the control. The expression of chondrogenic genes (SOX9, ACAN, COL2A1) in BMMSCs and chondrocytes were upregulated by Ppy and Ppy/Au NPs, as compared to controls. Histological staining with safranin-O indicated higher extracellular matrix production in Ppy and Ppy/Au NPs stimulated samples, as compared to controls. In conclusion, Ppy and Ppy/Au NPs stimulate BMMSC chondrogenic differentiation; however, BMMSCs were more responsive to Ppy, while chondrocytes possessed a stronger chondrogenic response to Ppy/Au NPs.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2023
CC license CC license description