| Abstract [eng] |
Taking into account all the challenges of tumorous tissue diagnostics there is an obvious need for portable, real time and reliable spectroscopic system capable of tissue assessment performed under in situ and even under in vivo conditions. The main goal of the thesis is to adapt the fiber based ATR IR (attenuated total reflection Infrared) spectroscopy system for the application for biological tissue investigation suitable to perform under intraoperative conditions. During the study, fiber based spectroscopy system was applied for the identification of freshly resected normal and tumorous tissues of various organs (kidney, urinary bladder, pancreas and liver). During the study, it was established that spectroscopic system based on fiber ATR IR probe coupled with conventional portable spectrometer and external MCT detector, is sensitive enough for collecting informative spectra of freshly resected tissues. Spectra, collected using fiber-based set-up, provides characteristic properties of different tissue types. Spectral markers such as glycogen, collagen and fructose 1,6-bisphosphatase can be used as spectral markers for differentiation of normal and tumorous tissues. However, there is no universal spectral tumor marker suitable for cancer detection in all human organs. The specific set of spectral markers for tumorous tissue identification should be established for each organ. Fiber-based ATR IR spectroscopy combined with appropriate statistical analysis methods applied for various human organ (kidney, bladder, pancreas and liver) tissues allows successful differentiation of normal and tumorous tissue up to 100 %. |
