| Abstract [eng] |
Solid keratin-rich waste management is one of essential research area in nowadays. Conventional chemical and high thermal keratin waste decomposition methods are fully explored and not enough effective for future biotechnolgy perspectives. However, traditional keratin-rich waste decomposition methods could be replaced by environmentally-friendly and economical microbial keratin waste biodegradation methods without energy wastage and essential amino acids and nutrition elements loss. Therefore, microbial bio-decomposition are attractive approach to keratin or keratin-like waste manage without any nutrition loss in eco-friendly process environment. Increased attention has been diverted to these keratinolytic peptidases because of their important potential uses in biomedicine, pharmaceutics, cosmetics and keratin waste bioconversion industries associated to the hydrolysis of keratin. Keratinolytic proteinases are next generation molecular tools for keratin hydrolysis and production of small value-added bio-active peptides. For the first time, we identified, purified and characterized native keratinolytic peptidase (BtKER) from Bacillus thuringiensis AD-12. For the first time, we created chimeric (SynKer-TT and SynKer-TM) keratinolytic peptidases with improved physicochemical properties. Eventually, we demonstrated that metabolically active secretomes from keratinolytic microorganisms can be used for eco-friendly enzymatic keratin waste management in lab-scale system. |
