| Abstract [eng] |
The combination of chemotherapy and photodynamic therapy looks very promising when the cytostatic drugs and PDT affect different cellular targets. However, the cellular response to the treatment must be considered, as it can be favourable for tumour re-growth. The objective of the dissertation work was to investigate the expression of the cytokines VEGF and IL-1 following the combined treatment of PDT and cytostatic drugs in cancer cells in vitro and in murine tumours in vivo. It was found that 1) the effect of the mTHPC-mediated photodynamic treatment (PDT) in combination with doxorubicin (Dox) on viability of human epidermoid carcinoma A-431 and murine lung carcinoma LLC1 had an additive character; the character of the effect of mTHPC-mediated photodynamic treatment in combination with taxotere (Tax) on viability of A-431 cells was dose-dependent; 2) mTHPC-mediated PDT, single and in combination with Dox or Tax, stimulates the expression of vascular endothelial growth factor (VEGF) and interleukin 1 (IL-1) in vitro 3) mTHPC-mediated PDT induces the expression of VEGF and IL-1 in LLC1 tumours. |
