| Title |
Advanced preparation of fragment libraries enabled by oligonucleotide-modified 2 ',3 '-dideoxynucleotides / |
| Authors |
Medžiūnė, Justina ; Kapustina, Žana ; Žeimytė, Simona ; Jakubovska, Jevgenija ; Sindikevičienė, Rūta ; Čikotienė, Inga ; Lubys, Arvydas |
| DOI |
10.1038/s42004-022-00649-9 |
| Full Text |
|
| Is Part of |
Communications chemistry.. Berlin : Springer Nature. 2022, vol. 5, iss. 1, art. no. 34, p. [1-8].. ISSN 2399-3669 |
| Abstract [eng] |
The ever-growing demand for inexpensive, rapid, and accurate exploration of genomes calls for refinement of existing sequencing techniques. The development of next-generation sequencing (NGS) was a revolutionary milestone in genome analysis. While modified nucleotides already were inherent tools in sequencing and imaging, further modification of nucleotides enabled the expansion into even more diverse applications. Herein we describe the design and synthesis of oligonucleotide-tethered 2′,3′-dideoxynucleotide (ddONNTP) terminators bearing universal priming sites attached to the nucleobase, as well as their enzymatic incorporation and performance in read-through assays. In the context of NGS library preparation, the incorporation of ddONNTP fulfills two requirements at once: the fragmentation step is integrated into the workflow and the obtained fragments are readily labeled by platform-specific adapters. DNA polymerases can incorporate ddONNTP nucleotides, as shown by primer extension assays. More importantly, reading through the unnatural linkage during DNA synthesis was demonstrated, with 25-30% efficiency in single-cycle extension. |
| Published |
Berlin : Springer Nature |
| Type |
Journal article |
| Language |
English |
| Publication date |
2022 |
| CC license |
|
