| Abstract [eng] |
This work discusses metal and metal oxide nanostructure application for protein analyte biosensing in optical and/or piezoelectric methods. Out of all summarized, porous aluminium oxide, zinc oxide nanowires and thin gold films were identified to be in possession of suitable chemical properties, such as stability and biocompatibility. This work includes methods of nanostructure synthesis, functionalization and surface application towards biosensing. This work introduces utilised measurement methods of spectroscopic ellipsometry, total internal reflection ellipsometry and hybrid method of spectroscopic ellipsometry/quartz crystal microbalance with dissipation. Following research results were presented: analysed human serum albumin nonspecific adsorption into porous anodic aluminium oxide nanopores. Applied electrochemically formed zinc oxide nanowires for bovine serum albumin immobilization, quantitively analysed the diffusion impact for protein immobilization. Thin gold films were applied for SARS-CoV-2 spike protein and its’ α and β variant immobilization for immune complex formation with specific antibodies, immune complex formation kinetic parameters were quantitively determined. Hybrid spectroscopic ellipsometry/quartz crystal microbalance method was used to analyse interaction between SARS-CoV-2 nucleocapsid protein and specific antibodies, spatial arrangement of proteins in an immune complex was determined using a geometric model. |
