Title Menstrual blood-derived stem cell paracrine factors possess stimulatory effects on chondrogenesis in vitro and diminish the degradation of articular cartilage during osteoarthritis /
Authors Uzielienė, Ilona ; Bialaglovytė, Paulina ; Mikšiūnas, Rokas ; Lebedis, Ignas ; Pachaleva, Jolita ; Vaičiulevičiūtė, Raminta ; Ramanavičienė, Almira ; Kvederas, Giedrius ; Bernotienė, Eiva
DOI 10.3390/bioengineering10091001
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Is Part of Bioengineering: Osteoarthritis and Cartilage Tissue Repair).. Basel : MDPI. 2023, vol. 10, iss. 9, art. no. 1001, p. 1-17.. eISSN 2306-5354
Keywords [eng] menstrual blood mesenchymal stem cells ; bone marrow mesenchymal stem cells ; chondrogenic differentiation ; TGF-β3 ; activin A ; BMP-2 ; IGF-1 ; cartilage ; paracrine effects
Abstract [eng] Articular cartilage is an avascular tissue with a limited capacity for self-regeneration, leading the tissue to osteoarthritis (OA). Mesenchymal stem cells (MSCs) are promising for cartilage tissue engineering, as they are capable of differentiating into chondrocyte-like cells and secreting a number of active molecules that are important for cartilage extracellular matrix (ECM) synthesis. The aim of this study was to evaluate the potential of easily accessible menstrual blood-derived MSC (MenSC) paracrine factors in stimulating bone marrow MSC (BMMSCs) chondrogenic differentiation and to investigate their role in protecting cartilage from degradation in vitro. MenSCs and BMMSCs chondrogenic differentiation was induced using four different growth factors: TGF-β3, activin A, BMP-2, and IGF-1. The chondrogenic differentiation of BMMSCs was stimulated in co-cultures with MenSCs and cartilage explants co-cultured with MenSCs for 21 days. The chondrogenic capacity of BMMSCs was analyzed by the secretion of four growth factors and cartilage oligomeric matrix protein, as well as the release and synthesis of cartilage ECM proteins, and chondrogenic gene expression in cartilage explants. Our results suggest that MenSCs stimulate chondrogenic response in BMMSCs by secreting activin A and TGF-β3 and may have protective effects on cartilage tissue ECM by decreasing the release of GAGs, most likely through the modulation of activin A related molecular pathway. In conclusion, paracrine factors secreted by MenSCs may turn out to be a promising therapeutical approach for cartilage tissue protection and repair.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2023
CC license CC license description