| Title |
Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa / |
| Authors |
Selhorst, Philippe ; Lequime, Sebastian ; Dudas, Gytis ; Proesmans, Sam ; Lutumba, Pascal ; Katshongo, Freddy ; Ramadan, Kadrie ; Micalessi, Isabel ; Ahuka-Mundeke, Steve ; Vanlerberghe, Veerle ; Van Esbroeck, Marjan ; Ariën, KevinK |
| DOI |
10.1016/j.ijid.2023.04.391 |
| Full Text |
|
| Is Part of |
International journal of infectious diseases.. Oxford : Elsevier B.V.. 2023, vol. 133, p. 46-52.. ISSN 1201-9712. eISSN 1878-3511 |
| Keywords [eng] |
Africa ; Dengue ; DENV ; Phylogenetics ; Whole genome sequencing |
| Abstract [eng] |
Objectives: The origin and spread of dengue virus (DENV) circulating in Africa remain poorly characterized, with African sequences representing <1% of global sequence data. Methods: Whole genome sequencing was performed on serum samples (n = 29) from an undifferentiated fever study in 2016 in the Democratic Republic of Congo (DRC), and from febrile travelers returning from Africa. The evolutionary history of the newly acquired African DENV-1 (n = 1) and cosmopolitan genotype DENV-2 (n = 18) genomes was reconstructed using a phylogeographic, time-scaled Bayesian analysis on a curated DENV panel including all known African sequences. Results: A minimum of 10 and eight introductions could be identified into Africa for DENV-1 and cosmopolitan DENV-2, respectively, almost all originating from Asia. Three introductions were previously unknown. The currently circulating virus comprises mainly the recently introduced clades and one long-established African clade. Robust geographical clustering suggests limited spread of DENV after each introduction. Our data identified the DRC as the source of the 2018 Angolan DENV-2 epidemic, and similarly, the 2013 Angolan DENV-1 outbreak as the origin of our DRC study. Conclusion: Active genomic surveillance of DENV in Africa at the portals of entry might help early outbreak response and limit sero- and genotype spread and human disease burden. |
| Published |
Oxford : Elsevier B.V |
| Type |
Journal article |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
