Title Methods gold standard in clinic millifluidics multiplexed extended gate field-effect transistor biosensor with gold nanoantennae as signal amplifiers /
Authors Janićijević, Željko ; Nguyen-Le, Trang-Anh ; Alsadig, Ahmed ; Cela, Isli ; Žilėnaitė, Rugilė ; Tonmoy, Taufhik Hossain ; Kubeil, Manja ; Bachmann, Michael ; Baraban, Larysa
DOI 10.1016/j.bios.2023.115701
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Is Part of Biosensors and bioelectronics.. Oxford : Elsevier Ltd. 2023, vol. 241, art. no. 115701, p. [1-11].. ISSN 0956-5663. eISSN 1873-4235
Keywords [eng] electrochemical impedance spectroscopy ; extended gate field-effect transistor ; gold nanoparticle bioconjugates ; immunosensor ; multiplexing ; potentiometric response
Abstract [eng] We present a portable multiplexed biosensor platform based on the extended gate field-effect transistor and demonstrate its amplified response thanks to gold nanoparticle-based bioconjugates introduced as a part of the immunoassay. The platform comprises a disposable chip hosting an array of 32 extended gate electrodes, a readout module based on a single transistor operating in constant charge mode, and a multiplexer to scan sensing electrodes one-by-one. Although employing only off-the-shelf electronic components, our platform achieves sensitivities comparable to fully customized nanofabricated potentiometric sensors. In particular, it reaches a detection limit of 0.2 fM for the pure molecular assay when sensing horseradish peroxidase-linked secondary antibody (∼0.4 nM reached by standard microplate methods). Furthermore, with the gold nanoparticle bioconjugation format, we demonstrate ca. 5-fold amplification of the potentiometric response compared to a pure molecular assay, at the detection limit of 13.3 fM. Finally, we elaborate on the mechanism of this amplification and propose that nanoparticle-mediated disruption of the diffusion barrier layer is the main contributor to the potentiometric signal enhancement. These results show the great potential of our portable, sensitive, and cost-efficient biosensor for multidimensional diagnostics in the clinical and laboratory settings, including e.g., serological tests or pathogen screening.
Published Oxford : Elsevier Ltd
Type Journal article
Language English
Publication date 2023
CC license CC license description