| Title |
The impact of acute nutritional interventions on the plasma proteome / |
| Authors |
Vernardis, Spyros I ; Demichev, Vadim ; Lemke, Oliver ; Grüning, Nana-Maria ; Messner, Christoph ; White, Matt ; Pietzner, Maik ; Peluso, Alina ; Collet, Tinh-Hai ; Henning, Elana ; Gille, Christoph ; Campbell, Archie ; Hayward, Caroline ; Porteous, David J ; Marioni, Riccardo E ; Mülleder, Michael ; Železniak, Aleksej ; Wareham, Nicholas J ; Langenberg, Claudia ; Farooqi, I Sadaf ; Ralser, Markus |
| DOI |
10.1210/clinem/dgad031 |
| Full Text |
|
| Is Part of |
The journal of clinical endocrinology and metabolism.. Washington : Oxford University Press. 2023, vol. 108, iss. 8, p. 2087-2098.. ISSN 0021-972X. eISSN 1945-7197 |
| Keywords [eng] |
APOC1 ; caloric restriction ; oral glucose tolerance test ; plasma proteomics ; type 2 diabetes |
| Abstract [eng] |
CONTEXT: Humans respond profoundly to changes in diet, while nutrition and environment have a great impact on population health. It is therefore important to deeply characterize the human nutritional responses. OBJECTIVE: Endocrine parameters and the metabolome of human plasma are rapidly responding to acute nutritional interventions such as caloric restriction or a glucose challenge. It is less well understood whether the plasma proteome would be equally dynamic, and whether it could be a source of corresponding biomarkers. METHODS: We used high-throughput mass spectrometry to determine changes in the plasma proteome of i) 10 healthy, young, male individuals in response to 2 days of acute caloric restriction followed by refeeding; ii) 200 individuals of the Ely epidemiological study before and after a glucose tolerance test at 4 time points (0, 30, 60, 120 minutes); and iii) 200 random individuals from the Generation Scotland study. We compared the proteomic changes detected with metabolome data and endocrine parameters. RESULTS: Both caloric restriction and the glucose challenge substantially impacted the plasma proteome. Proteins responded across individuals or in an individual-specific manner. We identified nutrient-responsive plasma proteins that correlate with changes in the metabolome, as well as with endocrine parameters. In particular, our study highlights the role of apolipoprotein C1 (APOC1), a small, understudied apolipoprotein that was affected by caloric restriction and dominated the response to glucose consumption and differed in abundance between individuals with and without type 2 diabetes. CONCLUSION: Our study identifies APOC1 as a dominant nutritional responder in humans and highlights the interdependency of acute nutritional response proteins and the endocrine system. |
| Published |
Washington : Oxford University Press |
| Type |
Journal article |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
